Utilizing isotope dilution-matrix-assisted laser desorption ionization-time of flight mass spectrometry as a reference procedure for the radioimmunoassay of serum thyroxine

Yu Chang Tyan, Shiang Bin Jong, Ming Hui Yang, Tsung Lin Wu, Tze Wen Chung, Pao-Chi Liao, Chia Yang Lin, Ying Fong Huang

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Background: The thyroid hormone, thyroxine (T4), is a tyrosine-based hormone produced by the thyroid gland, which is essential in regulating a number of biological processes, including growth, neurodevelopment, carbohydrate metabolism, oxygen consumption and protein synthesis. Data on human thyroid hormone metabolism were gathered since the middle of the 1970s mainly by the use of radioactive iodinated (125I or 131I) hormones. Methods: We describe an isotope dilution-matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method for the simultaneous determination of endogenous thyroid hormone and its 13C6-labeled analogue in serum. The 13C6-thyroxine (13C6-T4) was used as an internal standard; T4 and its isotopically labeled analogue were measured in the selected reaction monitoring mode for the transitions from m/z 777.8 to 732.1 and from m/z 784.2 to 738.1, respectively. Results: Serum samples were prepared and concentrated by a solid-phase extraction chromatography method. The recovery rate was measured by 125I-T4 and can be up to 82.8±2.8%. The detection limit and linear range for T4 were 5ng/ml and 5-400ng/ml, respectively. The correlation coefficient (R2) between radioimmunoassay (RIA) and isotope dilution-MALDI-TOF MS for detection of serum T4 was 0.982. Conclusion: This assay has a good relationship against a commercial RIA and the isotope dilution-mass spectrometry method and may serve as a reference method for quantitative analysis of T4.

原文English
頁(從 - 到)99-103
頁數5
期刊Clinica Chimica Acta
420
DOIs
出版狀態Published - 2013 一月 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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