Vaccination with the glycoprotein D gene of pseudorabies virus delivered by nonpathogenic Escherichia coli elicits protective immune responses

Ai Li Shiau, Chun Yen Chu, Wen Cheng Su, Chao Liang Wu

研究成果: Article同行評審

19 引文 斯高帕斯(Scopus)

摘要

Attenuated intracellular bacteria, such as Salmonella and Shigella, have been exploited to act as gene delivery vectors. In this study, we report that nonpathogenic, live Escherichia coli can be used for the delivery of DNA vaccines in vivo, leading to generation of immune responses against plasmid-encoded foreign antigens. The pseudorabies virus (PrV) DNA vaccine carrying the glycoprotein D (gD) gene delivered by E. coli was able to induce protective immune responses in mice against a lethal PrV challenge. Co-delivery of E. coli carrying plasmid DNA encoding prothymosin α enhanced cellular immune responses to the PrV DNA vaccine delivered by E. coli. Our results suggest that nonpathogenic E. coli may be used as a vector for DNA vaccines in veterinary uses.

原文English
頁(從 - 到)3277-3284
頁數8
期刊Vaccine
19
發行號23-24
DOIs
出版狀態Published - 2001 4月 30

All Science Journal Classification (ASJC) codes

  • 分子醫學
  • 一般免疫學和微生物學
  • 一般獸醫學
  • 公共衛生、環境和職業健康
  • 傳染性疾病

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