Verification of necessity for bioaugmentation - Lessons from two batch case studies for bioremediation of diesel-contaminated soils

Pao Wen Grace Liu, Liang-Ming Whang, Tsung Chain Chang, I. Cheng Tseng, Po Tseng Pan, Sheng Shung Cheng

研究成果: Article同行評審

18 引文 斯高帕斯(Scopus)

摘要

Background: Two bio-pile studies undertaken in a controlled laboratory were aimed at deciding optimal strategies to remediate two different artificial diesel-contaminated soils. Bioaugmentation with various inoculations, biostimulation with various levels of biosurfactant rhamnolipid, and nutrient enhancementwere the proposed remediation courses. Results: In Case I, the average of the first-order kinetic degradation rate constants during the first degradation stage for the bioaugmentation/biostimulation treatmentswas 0.0195±0.0056 d-1,which was about 2.6-fold higher than that of the control batch (0.0075 d-1). Conversely, in Case II, the rate constants for treatments with amendments and those for the control batch were found to be comparable, 0.0172 ± 0.0015 d-1 and 0.0158 d-1, respectively. Microarray results indicated a less diverse indigenous bacterial community in Case I and an abundant indigenous community in Case II, both from the control batches on Day 0. The dynamics of the two microbial communities, revealed by NMS plots, emphasized the similarity among the different treatments during the first degradation stage. Conclusions: Prior to a remediation project, the usefulness of a bioaugmentation approach can be investigated using an ITS oligonucliotide microarray. Results from the microarray answered why the bioaugmentation approach was useful in Case I, but not in Case II. The abundance of the diesel-degrading community determined the usefulness of bioaugmentation. Relatively quantified TRFLP results analyzed via the NMS plots demonstrated comparable microbial communities during the first degradation stage, regardless of differences between the two batches. The bacterial community structure might shift with the availability of hydrocarbons.

原文English
頁(從 - 到)808-819
頁數12
期刊Journal of Chemical Technology and Biotechnology
84
發行號6
DOIs
出版狀態Published - 2009 6月 29

All Science Journal Classification (ASJC) codes

  • 生物技術
  • 化學工程 (全部)
  • 可再生能源、永續發展與環境
  • 燃料技術
  • 廢物管理和處置
  • 污染
  • 有機化學
  • 無機化學

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