TY - JOUR
T1 - Virulence diversity among bacteremic aeromonas isolates
T2 - Ex Vivo, animal, and clinical evidences
AU - Po-Lin, Chen
AU - Chi-Jung, Wu
AU - Pei-Jane, Tsai
AU - Hung-Jen, Tang
AU - Yin-Ching, Chuang
AU - Nan-Yao, Lee
AU - Ching-Chi, Lee
AU - Chia-Wen, Li
AU - Ming-Chi, Li
AU - Chi-Chung, Chen Hung Wen Tsai
AU - Chun-Chun, Ou
AU - Chang-Shi, Chen
AU - Wen-Chien, Ko
N1 - Publisher Copyright:
©2014 Chen et al.
PY - 2014/11/6
Y1 - 2014/11/6
N2 - Conclusions/Significance: Clinical data, ex vivo experiments, and animal studies suggest there is virulence variation among clinically important Aeromonas species.Methodology/Principal Findings: Nine of four species of Aeromonas blood isolates, including A. dhakensis, A. hydrophila, A. veronii and A. caviae were randomly selected for analysis. The species was identified by the DNA sequence matching of rpoD. Clinically, the patients with A. dhakensis bacteremia had a higher sepsis-related mortality rate than those with other species (37.5% vs. 0%, P = 0.028). Virulence of different Aeromonas species were tested in C. elegans, mouse fibroblast C2C12 cell line and BALB/c mice models. C. elegans fed with A. dhakensis and A. caviae had the lowest and highest survival rates compared with other species, respectively (all P values〈0.0001). A. dhakensis isolates also exhibited more cytotoxicity in C2C12 cell line (all P values〈0.0001). Fourteen-day survival rate of mice intramuscularly inoculated with A. dhakensis was lower than that of other species (all P values 〈0.0001). Hemolytic activity and several virulence factor genes were rarely detected in the A. caviae isolates.The objective of this study was to compare virulence among different Aeromonas species causing bloodstream infections.
AB - Conclusions/Significance: Clinical data, ex vivo experiments, and animal studies suggest there is virulence variation among clinically important Aeromonas species.Methodology/Principal Findings: Nine of four species of Aeromonas blood isolates, including A. dhakensis, A. hydrophila, A. veronii and A. caviae were randomly selected for analysis. The species was identified by the DNA sequence matching of rpoD. Clinically, the patients with A. dhakensis bacteremia had a higher sepsis-related mortality rate than those with other species (37.5% vs. 0%, P = 0.028). Virulence of different Aeromonas species were tested in C. elegans, mouse fibroblast C2C12 cell line and BALB/c mice models. C. elegans fed with A. dhakensis and A. caviae had the lowest and highest survival rates compared with other species, respectively (all P values〈0.0001). A. dhakensis isolates also exhibited more cytotoxicity in C2C12 cell line (all P values〈0.0001). Fourteen-day survival rate of mice intramuscularly inoculated with A. dhakensis was lower than that of other species (all P values 〈0.0001). Hemolytic activity and several virulence factor genes were rarely detected in the A. caviae isolates.The objective of this study was to compare virulence among different Aeromonas species causing bloodstream infections.
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U2 - 10.1371/journal.pone.0111213
DO - 10.1371/journal.pone.0111213
M3 - Article
C2 - 25375798
AN - SCOPUS:84909989293
SN - 1932-6203
VL - 9
JO - PloS one
JF - PloS one
IS - 11
M1 - e111213
ER -