TY - CHAP
T1 - Visualization of gram-positive bacterial pili
AU - Chang, Chungyu
AU - Huang, I. Hsiu
AU - Hendrickx, Antoni P.A.
AU - Ton-That, Hung
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - Pili or fimbriae are recognized as essential virulence determinants assembled on the bacterial surface. Gram-positive bacteria produce covalently linked pilus structures that are distinct from gram-negative counterparts. In this chapter, we describe three commonly used techniques to extract, detect, and visualize pili from gram-positive bacteria: (1) Western blot analysis, (2) Immuno-Electron Microscopy, and (3) Atomic Force Microscopy.
AB - Pili or fimbriae are recognized as essential virulence determinants assembled on the bacterial surface. Gram-positive bacteria produce covalently linked pilus structures that are distinct from gram-negative counterparts. In this chapter, we describe three commonly used techniques to extract, detect, and visualize pili from gram-positive bacteria: (1) Western blot analysis, (2) Immuno-Electron Microscopy, and (3) Atomic Force Microscopy.
UR - http://www.scopus.com/inward/record.url?scp=84879284497&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84879284497&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-245-2_5
DO - 10.1007/978-1-62703-245-2_5
M3 - Chapter
C2 - 23299729
AN - SCOPUS:84879284497
SN - 9781627032445
T3 - Methods in Molecular Biology
SP - 77
EP - 95
BT - Bacterial Cell Surfaces
PB - Humana Press Inc.
ER -