Visualization of gram-positive bacterial pili

Chungyu Chang, I. Hsiu Huang, Antoni P.A. Hendrickx, Hung Ton-That

研究成果: Chapter

15 引文 斯高帕斯(Scopus)

摘要

Pili or fimbriae are recognized as essential virulence determinants assembled on the bacterial surface. Gram-positive bacteria produce covalently linked pilus structures that are distinct from gram-negative counterparts. In this chapter, we describe three commonly used techniques to extract, detect, and visualize pili from gram-positive bacteria: (1) Western blot analysis, (2) Immuno-Electron Microscopy, and (3) Atomic Force Microscopy.

原文English
主出版物標題Bacterial Cell Surfaces
主出版物子標題Methods and Protocols
發行者Humana Press Inc.
頁面77-95
頁數19
ISBN(列印)9781627032445
DOIs
出版狀態Published - 2013

出版系列

名字Methods in Molecular Biology
966
ISSN(列印)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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