Widefield multiphoton microscopy with image-based adaptive optics

Chia-Yuan Chang, L. C. Cheng, H. W. Su, W. C. Yen, S. J. Chen

研究成果: Conference contribution


Unlike conventional multiphoton microscopy according to pixel by pixel point scanning, a widefield multiphoton microscope based on spatiotemporal focusing has been developed to provide fast optical sectioning images at a frame rate over 100 Hz. In order to overcome the aberrations of the widefield multiphoton microscope and the wavefront distortion from turbid biospecimens, an image-based adaptive optics system (AOS) was integrated. The feedback control signal of the AOS was acquired according to locally maximize image intensity, which were provided by the widefield multiphoton excited microscope, by using a hill climbing algorithm. Then, the control signal was utilized to drive a deformable mirror in such a way as to eliminate the aberration and distortion. A R6G-doped PMMA thin film is also increased by 3.7-fold. Furthermore, the TPEF image quality of 1 μm fluorescent beads sealed in agarose gel at different depths is improved.

主出版物標題Unconventional Imaging and Wavefront Sensing 2012
出版狀態Published - 2012 十二月 1
事件Unconventional Imaging and Wavefront Sensing 2012 - San Diego, CA, United States
持續時間: 2012 八月 132012 八月 14


名字Proceedings of SPIE - The International Society for Optical Engineering


ConferenceUnconventional Imaging and Wavefront Sensing 2012
國家/地區United States
城市San Diego, CA

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 凝聚態物理學
  • 電腦科學應用
  • 應用數學
  • 電氣與電子工程


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