WWOX suppresses prostate cancer cell progression through cyclin D1-mediated cell cycle arrest in the G1 phase

Jen Tai Lin, Hao Yi Li, Nan Shan Chang, Cheng Han Lin, Yu Chia Chen, Pei Jung Lu

研究成果: Article同行評審

29 引文 斯高帕斯(Scopus)

摘要

WW domain-containing oxidoreductase (WWOX) has been reported to be a tumor suppressor in multiple cancers, including prostate cancer. WWOX can induce apoptotic responses to inhibit tumor progression, and the other mechanisms of WWOX in tumor suppression have also been reported recently. In this study, we found significant down-regulation of WWOX in prostate cancer specimens and prostate cancer cell lines compared with the normal controls. In addition, an ectopically increased WWOX expression repressed tumor progression both in vitro and in vivo. Interestingly, overexpression of WWOX in 22Rv1 cells led to cell cycle arrest in the G1 phase but did not affect sub-G1 in flow cytometry. GFP-WWOX overexpressed 22Rv1 cells were shown to inhibit cell cycle progression into mitosis under nocodazole treatment in flow cytometry, immunoblotting and GFP fluorescence. Further, cyclin D1 but not apoptosis correlated genes were down-regulated by WWOX both in vitro and in vivo. Restoration of cyclin D1 in the WWOXoverexpressed 22Rv1 cells could abolish the WWOX-mediated tumor repression. In addition, WWOX impair c-Junmediated cyclin D1 promoter activity. These results suggest that WWOX inhibits prostate cancer progression through negatively regulating cyclin D1 in cell cycle lead to G1 arrest. In summary, our data reveal a novel mechanism of WWOX in tumor suppression.

原文English
頁(從 - 到)408-416
頁數9
期刊Cell Cycle
14
發行號3
DOIs
出版狀態Published - 2015 二月 1

All Science Journal Classification (ASJC) codes

  • 分子生物學
  • 發展生物學
  • 細胞生物學

指紋

深入研究「WWOX suppresses prostate cancer cell progression through cyclin D1-mediated cell cycle arrest in the G1 phase」主題。共同形成了獨特的指紋。

引用此