AC Electrokinetic Manipulation for Separation and in situ Detection of Cancer Cells

摘要

Recently personalized cancer treatment had been developed vigorously However there are many problems in traditional isolation and detection methods such as low purity complicated operation procedures and expensive instruments Thus how to separate tumor cells from clinical samples rapidly and precisely and how to improve the efficient diagnosis for a proper reference of personalized treatment are the aims of this study AC electrokinetics for the manipulation of cells had been widely integrated into microfluidic chips and biosensors for manipulating and analyzing specific targets accurately In this study dielectrophoresis and electrorotation were integrated into our novel biochip platform to achieve separation of tumor cells capture of single tumor cell and analysis of drug response in the tumor cell at the same reaction In this thesis three subjects will be introduced (1) Separation of cancer cells from complex samples by electrokinetics: We developed a new on-chip separation method by different dielectric properties cancer cells were transported to specific detection area and non-cancer cells were excluded out of detection area as an AC electric field was applied to each electrodes (2) Capture of single cancer cell by a switch circuit and three-dimensional dielectrophoresis: When single cancer cell entered into detection area the upper electrodes were turned on and generated a strong electric field with under electrodes to block extra cancer cells That single cell would be tracked to the detection center with a relatively low electric field The efficiency to capture single cell is up to 85% within one min (3) Assessment of cancer cells’ electrical characteristics by spectrum of three-dimensional electrorotation (ROT): The results of ROT spectrum can be achieved rapid assessment of cancer cells’ electrical characteristic in which the variations can be reduced obviously and the high frequency of spectrum can be analyzed more completely than traditional ROT method by combining an extra-stabilized system into our device As a result the optimal frequency for electrorotation discrimination of cancer cell was found at 100 kHz and the total detection time is within 5 min per cell Finally we developed a miniaturized and integrated biochip that can be used in samples pretreatment and detection of cancer cells successively We hope this platform can be used widely in personalized cancer treatment because of its convenient timesaving and low cost

獎項日期	2014 8月 21
原文	English
監督員	Hsien-Chang Chang (Supervisor)