Analysis of host T-cell response in diffuse large B-cell lymphoma (DLBCL) and correlation with clinicopathological features

  • 張 珍

Student thesis: Master's Thesis

Abstract

Abstract 1 Objectives Immune system plays an important role to eliminate cancer cells Cancer immunity is a complex mechanism and shows potentials for therapeutic targets As a traditional model tumor antigens captured and processed by dendritic cells would be presented to effector/memory T cells and cytotoxic T cells which then recognize and attack tumor cells Regulatory T cells however would repress the other immune cells to prevent excess tissue destruction Unlike solid tumors lymphomas are originated from immune system which might directly influence host immune system and reveal more unpredictable results Diffuse large B-cell lymphoma (DLBCL) is an aggressive B-cell lymphoma and is the most common type of adult lymphoma Analysis of host immune cells may provide useful information for assessing prognosis or possibly clinical management In addition poor immune surveillance is expected to influence on tumor progression In human beings brain is an immune-privileged sanctuary which may contribute to an ineffective host anti-immune response Therefore we focused on a specific DLBCL subtype-primary central nervous system (CNS) diffuse large B-cell lymphoma (PCNSL) and compared it to peripheral counterpart 2 Methods Peripheral blood samples from 77 DLBCL patients and 30 healthy volunteers were analyzed using flow cytometry immunophenotyping Complete blood cell counts T cell subsets and dendritic cells (DCs) were detected and the results were correlated with clinicopathologic characteristics In addition thirty-two biopsy specimens of PCNSL and 30 specimens of low-stage non-CNS DLBCL from immunocompetent patients formed the study group The density and distribution of immune cells including dendritic cells (DC-LAMP+ and S100+) effector/memory T-cells (CD45RO+) and cytotoxic T-cells (granzyme B+) and expression of HLA-DR by lymphoma cells were evaluated immunohistochemically 3 Results Compared with healthy volunteers DLBCL patients had significantly higher leukocyte and monocyte counts (P
Date of Award2015 七月 10
語言English
SupervisorKung-Chao Chang (Supervisor)

引用此文

Analysis of host T-cell response in diffuse large B-cell lymphoma (DLBCL) and correlation with clinicopathological features
珍, 張. (Author). 2015 七月 10

Student thesis: Master's Thesis