Characterization of glycosylation patterns of interleukin-6 between non-small lung cancer cell lines and macrophage

摘要

We previously reported that NSCLC patients with high circulating interleukin-6 (IL-6) levels had survival outcomes inferior to those of NSCLC patients with low circulating IL-6 levels Therefore circulating IL-6 is considered a prognostic factor for patients with NSCLC In lung cancer patients the circulating IL-6 can be secreted from tumor cells inflammatory cells or others Because cell-type-specific glycosylation patterns of IL-6 have been detected in previous studies we wanted to identify the specific glycosylation patterns of IL-6 in lung cancer cells With these findings we then will be able to delineate the sources of circulating IL-6 and develop biomarkers for early diagnosis and predictive markers for treatment response in lung cancer patients In this study we examined the glycosylation patterns of IL-6 produced from various lung cancer lines cell lines with specific activated oncogenes and cells from clinical specimens of lung cancer patients We then compared IL-6 patterns to those of macrophages We also amplified lung cancer cell lines and macrophages to collect their conditioned medium and then tried different experimental conditions to purify 1 ?g of IL-6 for analysis We use LC-MS/MS and lectin array to analyze IL-6 Additionally we planned to develop a method for detecting circulating IL-6 with specific glycosylation patterns We then hope to be able to evaluate the potential of using lung cancer-specific IL-6 glycoforms as novel biomarkers for early diagnosis and prediction of treatment response in lung cancer patients

獎項日期	2016 2月 2
原文	English
監督員	Wu-Chou Su (Supervisor)