Hyaluronan has been documented in important biological processes In our lab’s previous studies cells cultured on coated hyaluronan (CHA) substratum exhibited reduced proliferation rate yet still maintained the differentiation potentials specific for mesenchymal stem cells Some researchers were conducted on the effects of high molecular weight hyaluronan on maintaining stem cell characteristics yet no study has reported the effects of long-term hyaluronan treatment on stem cells Our study aims at elucidating the effect of hyaluronan on placenta-derived mesenchymal stem cells (PDMSCs) cultured in CHA versus TCS conditions First we characterized and established biomarkers expression profile of PDMSC For comparison of hyaluronan-mediated protein differential expression in TCS versus CHA time points were selected for synchronized cell cycle phase on TCS and CHA culture conditions Cellular proteins were labeled using stable isotope labeling with amino acids in cell culture (SILAC) followed by protein identification protein quantification and ratio determination by using liquid chromatography tandem-mass spectrometry (LC-MS/MS) Finally in silica prediction of differentially displayed protein patterns was generated In our findings in PDMSC long-term cultured on TCS Ras protein and its related signaling pathway components MAP2K3;MAP2K6 and actin-interacting proteins (RND3 LIMA1 FHL1 NEFM TPM1 and FLNC) were up-regulated and may be correlated to stress fiber formation and possibly promoted senescence and aging In contrary CHA long-term culture was correlated with down-regulation of Ras signaling and related proteins and up-regulation of NAMPT and molecules from the endoplasmic reticulum-associated degradation (ERAD) pathway Notably NAMPT up-regulation may be associated with degradation of excess p53 and thus prevented p53-mediated senescence and maintained normal phenotype of PDMSC
Effect of Hyaluronan on Placenta-Derived Mesenchymal Stem Cells
映卉, 陳. (Author). 2016 7月 27
學生論文: Doctoral Thesis