Effects of transcutaneous electrical nerve stimulation (TENS) on diabetic neuropathic pain in rats

論文翻譯標題: 經皮神經電刺激對大鼠糖尿病神經病變性疼痛之影響
  • 賴 奕儒

學生論文: Master's Thesis


Background and Purpose: Transcutaneous electrical nerve stimulation (TENS) has been used to improve heat and cold pain thresholds and decrease the pain scales in diabetic patients However it remains unknown whether TENS could attenuate diabetic neuropathy Previous research has shown that hyperglycemia could change the levels of TNF-? TNFR1 TNFR2 cleaved caspase-3 p-Akt and p-PI3k in the PN Thus this study investigates the effects of TENS in the pain-like behaviors of the diabetic rats and the expression of TNF-? TNFR1 TNFR2 cleaved caspase-3 p-Akt and p-PI3k in the DRG SCDH SN and PN Methods: Five experimental groups consist of control STZ STZ+ HF TENS STZ+ LF TENS and STZ+ AF TENS TENS will be delivered to the HF LF and AF groups for 30 minutes per day and 5 days per week for 3 weeks The mechanical and thermal pain thresholds have been tested under the rats’ hind paws of each side The rats were sacrificed on the 21st day since the diabetic induction After the 30-minute post-test period of the last treatment the L4-L6 SCDH DRGs SN and PN (including tibial nerve peroneal nerve and sural nerve) are collected for protein analysis TNF-? levels were detected by Enzyme-linked Immunosorbent Assay (ELISA) and TNFR1 TNFR2 cleaved caspase-3 p-Akt and p-PI3k expressions were analyzed by Western Blot Results: The peaks of the mechanical withdrawal threshold and thermal withdrawal latency are shown lasting after 30 minutes until 2 hours of treatment but they slightly decreased after 24 hours TENS can effectively increase the mechanical withdrawal threshold for 3 weeks However HF TENS only increases the thermal withdrawal latency for 3 weeks TNF-? level in the spinal cord dorsal horn and dorsal root ganglions were suppressed after the 3-week TENS treatment but it increased in the sciatic nerve and the peripheral nerve in both high and LF groups TNF-? level in the sciatic nerve only decreased in the AF group Expression of TNFR1 TNFR2 cleaved caspase-3 p-Akt and p-PI3k in the spinal cord dorsal horn significantly up-regulated in the STZ and treatment groups Expressions of TNFR1 TNFR2 and p-Akt in dorsal root ganglion up-regulated in the STZ and treatment groups as well However only the expressions of TNFR2 and p-PI3k in sciatic nerve increased in the STZ group The expressions of TNFR1 and TNFR2 in sciatic nerve and TNFR1 in peripheral nerve both enhanced in the treatment groups Conclusion: HF LF and AF TENS can all lower mechanical allodynia in diabetes but the decrease in TNF-? level and cell proliferation and the increase of apoptosis in LF and AF groups are more significant than HF group
獎項日期2015 7月 31
監督員Ching-Hsia Hung (Supervisor)