Fibronectin assembly-related miR-146a suppresses motility and metastasis through targeting vimentin in esophageal squamous cell carcinoma

  • 李 季樺

學生論文: Master's Thesis

摘要

MicroRNAs (miRNAs) are small non-coding RNAs which bind to 3’UTR of the target gene to degrade mRNA or inhibit translation MiRNA acts as either a tumor suppressor or an oncogene Esophageal cancer is the fifth leading cause of cancer death worldwide and ranks the ninth in Taiwan in 2013 There are two major histological types of esophageal cancers: Esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EA) Over 95% of the esophageal cancers in Taiwan are ESCC The major risk factor of ESCC is the metastatic spread of tumor cells which results in poor prognosis and low 5-year survival rate (less than 15%) However the underlying mechanism of metastasis in ESCC remains unclear Fibronectin (FN) a multifunctional adhesive glycoprotein which assembles on the cell membrane contributes to carcinogenesis including cell migration and invasion We previously showed that the abundance of FN assembly on ESCC cell membrane is negatively correlated with miR-146a expression In this study we identified that vimentin which is a mesenchymal marker and involves in epithelial mesenchymal transition (EMT) is the target gene of miR-146a We further proved that miR-146a suppresses both migration and invasion of CE81FN+ cell through inhibiting vimentin by Real-Time PCR and transwell assays respectively The importance of miR-146a and its target gene vimentin in clinical specimens was demonstrated by IHC staining followed by statistical analysis MiR-146a mediated suppression of metastasis was also detected in another ESCC cell line (KYSE) In conclusion we reveal that FN assembly-negatively related miR-146a acts as a tumor suppressor to inhibit ESCC cell migration and invasion by targeting vimentin
獎項日期2014 八月 28
原文English
監督員Hsiao-Sheng Liu (Supervisor)

引用此

Fibronectin assembly-related miR-146a suppresses motility and metastasis through targeting vimentin in esophageal squamous cell carcinoma
季樺, 李. (Author). 2014 八月 28

學生論文: Master's Thesis