In Situ Investigation of Molecular Immobilization on Au Surface by Surface-Enhanced Infrared Absorption Spectroscopy

論文翻譯標題: 表面增顯紅外光吸收光譜現址偵測分子在金表面修飾
  • 吳 宜榛

學生論文: Master's Thesis


Covalent binding has been wildly used in biosensors and biomaterials With the vigorous development of biosensors covalent immobilization is getting more and more attention The process of forming covalent bonds on biosensors including: (1) forming a self-assembly monolayer (SAM) (2) activating the SAM with crosslinkers to form an active layer and then (3) covalent attachment of biomolecules However the immobilization parameters are still based on experience and vary from one study to another In this study 11-mercaptoundecanoic acid (MUA) was used to form SAM on gold surface activating the surface through EDC/NHS and then formed peptide with aminoferrocene (AmFc) Known for high sensitivity low background interference and in-situ observation attenuated total reflection surface enhanced infrared adsorption spectroscopy (ATR-SEIRAS) was used to investigate the immobilization process and then to find a relative well optimized parameters
獎項日期2016 八月 11
監督員Hsien-Chang Chang (Supervisor)