Mining for novel genes regulating rice development from the T-DNA insertion mutant population

論文翻譯標題: 由T-DNA插入突變種源中發掘調控水稻發育之新穎基因
  • 鄭 明龍

學生論文: Doctoral Thesis


Rice is the most important staple food in the world and supports over 50% of the human population Sequencing of the whole genome of rice was finished in 2005 which predicted at least 30 000 genes The sequence data provide good bioinformatics information for rice gene functional studies The Taiwan Rice Insertional Mutants (TRIM) population contains at least 100 000 T-DNA insertion mutant lines making this a valuable resource and efficient tool for rice gene discoveries By adopting a forward genetics approach we screened the TRIM population and found an interesting T-DNA insertion mutant WINDING 1 (WIN1) The WINDING 1 (WIN1) gene encodes a protein containing BTB and NPH3 domains and a coiled-coil motif which is homologous to the Arabidopsis NPH3 protein The rice homolog of NPH3 CPT1 has been shown to be important for coleoptile phototropism and regulation of lateral auxin transport WIN1 is mainly expressed in roots and leaf/root junctions Subcellular localization studies show that WIN1 is primarily associated with plasmodesmata along the horizontal edges of plasma membranes However upon ectopic expression WIN1 is distributed on all edges of leaf blade and sheath cells The excurvature side of the spiral leaf sheath in WIN1Act and WIN1-Ox plants contains more auxin and F-actin than the incurvature side indicating that WIN1 regulates F-actin organization and causes asymmetric auxin distribution This dissertation demonstrates the utility of using the genetic resources in the TRIM population for gene discovery and functional studies in rice
獎項日期2017 八月 16
監督員Hao-Jen Huang (Supervisor)