Modulation and Characterization of Nuclear Factor κB Inhibitor-alpha (IκB?) for Environmental Stress in Orange-spotted Grouper (Epinephelus coioides)

論文翻譯標題: 點帶石斑魚在環境緊迫下IκB?基因之調節與特性分析
  • 謝 季妤

學生論文: Master's Thesis

摘要

One of the important transcription factors in innate immunity NF-κB/IκB? complex which can translocate to nucleus to bind to the promoter and regulate pro-inflammatory cytokine gene to modulate immune responses and defeat pathogens protect fish from pathogens infection in water even without a sound adaptive immunity like mammals In this study the open reading frame cDNA of orange-spotted grouper (Epinephelus coioides) IκB? were cloned with 924 bp and encoded 308 amino acids From the real-time PCR analysis it showed that NF-κB and IκB? widely expressed in various organs while under nervous necrosis virus (NNV) infection they mainly expressed in brain head kidney and liver; however dissociated IκB? protein expressed in immune organs under NNV infection On the other hand the expression of p65 and p50 showed 9 6-fold and 6 2-fold up-regulated at short time and gradually down-regulated under low temperature while gradually up-regulated under high temperature However under high temperature as well as NNV-infected NF-κB and IκB? revealed delayed up-regulation compared to non-infected group Taken together in orange-spotted grouper NF-κB/IκB? complex regulates gene changes under NNV infection or changes in environmental temperature which in turn may inhibit excessive inflammatory responses and increase the survival of grouper Therefore the NF-κB/IκB? complex may play a significantly regulatory role in the innate immune mechanism of grouper against environmental stress
獎項日期2018 12月 28
原文English
監督員Tzong-Yueh Chen (Supervisor)

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