Role of Prothymosin ? in the Pathogenesis of Polycystic Ovary Syndrome

論文翻譯標題: 前胸腺素在多囊性卵巢症候群致病機轉中扮演的角色
  • 劉 宛艷

學生論文: Master's Thesis


Polycystic ovary syndrome (PCOS) is one of the endocrine disorders of the female reproductive system and affects 6%-10% of women worldwide Clinical diagnostic features of PCOS are infertility hyperandrogenism and polycystic ovary In addition 50%-70% of patients with PCOS show obese and insulin resistance Prothymosin ? (ProT) an acidic nuclear protein plays a role in the acetylation of histone and non-histone proteins by decreasing its association with histone deacetylase We have recently reported that overexpression of ProT contributes to the development of insulin resistance In the clinical samples we found that ProT levels were significantly higher in the PCOS group compared with the non-PCOS group The aim of this study was to investigate whether ProT is involved in the pathogenesis of PCOS Next we established a dehydroepiandrosterone (DHEA)-induced PCOS mouse model DHEA-treated mice exhibited many characteristics of the human condition including disrupted estrous cycles hyperandrogenism polycystic ovary overweight and insulin resistance as well as expressed higher levels of ProT in the ovarian and adipose tissues In addition ProT transgenic mice had more severe PCOS than wild-type mice after DHEA treatment Our microarray data from the lung tissues of ProT transgenic mice revealed downregulation of genes that are involved in the peroxisome proliferator activated receptor γ (PPARγ) signaling pathway Therefore we hypothesized that ProT may play a pivotal role in the pathogenesis of PCOS through PPARγ signaling We observed that ProT overexpression reduced the transactivation activity of PPARγ ProT could interact with PPARγ and reduce PPARγ activity by enhancing its acetylation Collectively these results suggest that ProT may inhibit PPARγ activation leading to PCOS in DHEA-treated mice This study may not only shed lights on the pathogenesis of PCOS but also provide novel therapeutic strategies for PCOS
獎項日期2016 八月 16
監督員Chao-Liang Wu (Supervisor)