The modulating effects of stress sex and age on early neurogenesis in mouse dentate gyrus

摘要

This study aimed to assess sex differences and age-related modulating effect on the stressor-altered cell proliferation and early neuronal differentiation Unpredicted foot shock followed by restraint in water was used as a stressor regimen and the number of cell proliferation and early neurogenesis in mouse dentate gyrus (DG) was counted Young (8-week-old) and aged (9-month-old) C57BL/6N mice were intraperitoneally injected with a single dose of bromodeoxyuridine (BrdU) (100 mg/kg) immediately before the stress regimen Bromodeoxyuridine staining was used to indicate new mitotic cells and doublecortin co-staining was used to reveal early differentiated neural progenitors Aged mice had lower baselines in the number of newly proliferated cells and neural progenitors compared to young mice regardless of sex Although young mice were insensitive to the stressor regimen aged female mice exhibited significant decreases in the number of new cell differentiation and early neurogenesis in response to such stress regimen The presence of young companions (8-week-old) or aged companions (9-month-old) potentiated the stressor effect by decreasing the basal number of newly cell proliferation or early neurogenesis in aged male mice These findings suggest that old females may be more sensitive to the modulating effects of stress on early neurogenesis in the dentate gyrus as compared to old males

獎項日期	2016 7月 15
原文	English
監督員	Lung Yu (Supervisor)